Protein chimerism: Novel source of protein diversity in humans adds complexity to bottom-up proteomics
نویسندگان
چکیده
منابع مشابه
Construction and Analysis of Tissue-Specific Protein-Protein Interaction Networks in Humans
We have studied the changes in protein-protein interaction network of 38 different tissues of the human body. 123 gene expression samples from these tissues were used to construct human protein-protein interaction network. This network is then pruned using the gene expression samples of each tissue to construct different protein-protein interaction networks corresponding to different studied ti...
متن کاملSupplemental Materials: A Statistical Framework for Protein Quantitation in Bottom-Up MS-based Proteomics
Let Yijs and xijs be the intensity and a vector of covariates, respectively, for protein i, peptide j, and sample s, i = 1, 2, . . . ,M , j = 1, 2, . . . ,mi, s = 1, 2, . . . , n. We assume the linear model Yijs = xijs βi + ijs, where ijs ∼ N(0, σij), and the variables are mutually independent. Note that, with an appropriate choice of the model matrix composed of the xijs, this model parameteri...
متن کاملA statistical framework for protein quantitation in bottom-up MS-based proteomics
MOTIVATION Quantitative mass spectrometry-based proteomics requires protein-level estimates and associated confidence measures. Challenges include the presence of low quality or incorrectly identified peptides and informative missingness. Furthermore, models are required for rolling peptide-level information up to the protein level. RESULTS We present a statistical model that carefully accoun...
متن کامل: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes
هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...
Milk Bottom-Up Proteomics: Method Optimization
Milk is a complex fluid whose proteome displays a diverse set of proteins of high abundance such as caseins and medium to low abundance whey proteins such as ß-lactoglobulin, lactoferrin, immunoglobulins, glycoproteins, peptide hormones, and enzymes. A sample preparation method that enables high reproducibility and throughput is key in reliably identifying proteins present or proteins respondin...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: PROTEOMICS
سال: 2012
ISSN: 1615-9853
DOI: 10.1002/pmic.201200371